| 摘要 |
A method for producing the diagnostic hyperimmune serum against avian metapneumovirus comprises the subcutaneous injection of antigen in the areas of femur and scapula of the rabbits in the volume of 1 mL. The blood is taken from heart in 14 days after the last immunization. The antigen of field isolate PV-3 of metapneumovirus is injected on Days 1, 8, 15, and 29 as a mixture with incomplete Freund adjuvant. In addition, the immunomodulating substance morpholinium 2-(5-(pyridyl-4-yl)-4-(2-methoxyphenyl)-1,2,4-triazol-3-ylthio)acetate in a volume of 1 mL intramuscularly. |
