METHOD FOR DIFFERENTIATION OF ADULT STEM CELL INTO SCHWANN CELL USING ELECTROMAGNETIC FIELD
摘要
PURPOSE: A method for differentiating adult stem cells into schwann cells is provided to effectively differentiate adult stem cells into schwann cells without an expensive differentiation medium. CONSTITUTION: A method for differentiating adult stem cells into schwann cells using an electromagnetic field comprises the steps positioning the adult stem cells in the electromagnetic field at a frequency of 1-200 Hz; and culturing the adult stem cells. The intensity of the electromagnetic field is 0.5-2 mT. The culturing comprises the steps of: culturing the adult stem cells in an alpha-MEM medium containing beta-mercaptoethanol for 12-36 hours; culturing the adult stem cells in an alpha-MEM medium containing trans-retinoic acid for 60-82 hours; and culturing the adult stem cells in an alpha-MEM medium containing forskolin for 7-14 days.
申请公布号
KR20130099633(A)
申请公布日期
2013.09.06
申请号
KR20120021285
申请日期
2012.02.29
申请人
DONGGUK UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION;SNU R&DB FOUNDATION
发明人
SEO, YOUNG KWON;PARK, JUNG KEUG;HAN, MI JUNG;LEE, JONG HO;YOON, HEE HOON;KIM, SOO CHAN;KIM, SUNG MIN