发明名称 |
PREPARATION METHOD OF ANTIGEN-IMMOBILIZED IMMUNOFLUORESCENCE SLIDE, AND IMMUNOFLUORESCENCE SLIDE PREPARED THEREBY |
摘要 |
A method of preparing an antigen-immobilized immuno-fluorescence slide, the method comprising: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody that specifically binds to a target protein, with streptavidin to label the antibody with a fluorescent nanoparticle; immuno-reacting the antibody by competitive mixing, assaying with a fluorescence camera, wherein the immobilizing of the C-reactive protein on the slide comprises: modifying the slide with 3-aminopropyltrimethoxysilane to prepare a modified slide; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein at a concentration of 0.01-0.5 mg/ml in a 30-70 mM phosphate buffer solution (pH 6.5-7.8) to prepare an antigen solution for immobilization; placing a petri dish comprising the slide on a spotting guide and spotting 1-100 µ„“ of the antigen solution on spotting points; and performing a reaction on the slide prepared as described above for 1-6 hours to immobilize the antigen, and an immune-fluorescence slide prepared by using the method. |
申请公布号 |
EP2541248(A4) |
申请公布日期 |
2013.09.04 |
申请号 |
EP20110747611 |
申请日期 |
2011.02.21 |
申请人 |
KOREA FOOD RESEARCH INSTITUTE |
发明人 |
KIM, NAM SOO;CHO, YOUNG JIN;KIM, CHONG TAI |
分类号 |
G01N33/533;G01N33/543 |
主分类号 |
G01N33/533 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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