Charge-switch nucleotides based method for sequencing of nucleic acids

摘要

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labelled NPs that exhibit charge-switching behaviour, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electricity sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.