发明名称 |
Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry |
摘要 |
The present invention relates to the use of post source decay (PSD) or collision induced dissociation (CID) direct tissue (DT) MALDI-TOF or DT-MALDI-TOF-TOF mass spectrographic identification of biological molecules in a tissue or cellular sample without the need for further protein extraction. This method provides for studying cells or tissues by direct tissue MALDI (DT-MALDI), thereby substituting in situ protein release for further protein extraction. Mass/intensity data was processed with Mascot© software interrogation of the NCBI database. These results are proof of principle that DT-MALDI, combined with bioinformatics, can directly identify proteins in cells and tissues from their mass spectra.
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申请公布号 |
US8525104(B2) |
申请公布日期 |
2013.09.03 |
申请号 |
US20100799576 |
申请日期 |
2010.04.27 |
申请人 |
PEVSNER PAUL;NAFTOLIN FREDERICK;MILLER DOUGLAS C.;HILLMAN DEAN;STALL BRIAN K.;WISHNIES STEVEN M.;NEW YORK UNIVERSITY |
发明人 |
PEVSNER PAUL;NAFTOLIN FREDERICK;MILLER DOUGLAS C.;HILLMAN DEAN;STALL BRIAN K.;WISHNIES STEVEN M. |
分类号 |
B01D59/44;H01J49/00;H01J49/40 |
主分类号 |
B01D59/44 |
代理机构 |
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地址 |
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