发明名称 |
Position dependent recognition of GNN nucleotide triplets by zinc fingers |
摘要 |
The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5'-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
|
申请公布号 |
US8524874(B2) |
申请公布日期 |
2013.09.03 |
申请号 |
US201313743204 |
申请日期 |
2013.01.16 |
申请人 |
SANGAMO BIOSCIENCES, INC.;SANGAMO BIOSCIENCES |
发明人 |
LIU QIANG;REBAR EDWARD J.;JAMIESON ANDREW C. |
分类号 |
C07H21/02;C07K1/00;C07K14/00;C07K14/47;C12N9/64;C12N15/10;C12P21/02;C12Q1/68;G01N33/48;G01N33/50;G01N33/53;G01N33/574;G01N33/68;G06F19/00 |
主分类号 |
C07H21/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|