发明名称 |
siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof |
摘要 |
Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
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申请公布号 |
US8524681(B2) |
申请公布日期 |
2013.09.03 |
申请号 |
US20100727336 |
申请日期 |
2010.03.19 |
申请人 |
PURI NITIN;CHARLES IRUDAYA;MAGDALENO SUSAN;VLASSOV ALEXANDER;BURNETT CHRIS;APPLIED BIOSYSTEMS, LLC |
发明人 |
PURI NITIN;CHARLES IRUDAYA;MAGDALENO SUSAN;VLASSOV ALEXANDER;BURNETT CHRIS |
分类号 |
C07H21/02;A61K31/70;C07H21/04;C12N15/11;C12N15/113;C12Q1/68 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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