| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for producing recombinant viral vectors at high titers incorporating a variety of important advancement over the conventional art.SOLUTION: The problem is solved by incorporating multiple features which provide enhanced production of viruses, particularly those viruses encoding exogenous transgenes. The specifically illustrated method describes a method of the high titer serum-free media production of recombinant replication defective adenoviruses containing an exogenous transgene. The invention provides methods for preparing microcarriers, methods for seeding bioreactors at high cell density, increasing the infectivity of producer cells to the virus, methods for increasing product yield through synchronization of the cell cycle of the producer cells, and methods for minimizing the deleterious effects of exogenous transgenes. |
