Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.
申请公布号
EP1828385(B1)
申请公布日期
2013.08.28
申请号
EP20050854909
申请日期
2005.12.21
申请人
E.I. DU PONT DE NEMOURS AND COMPANY
发明人
PAYNE, MARK, S.;DICOSIMO, ROBERT;THOMPSON, JEFFERY, SCOTT;O'KEEFE, DANIEL, P.;PANOVA, ANNA