发明名称 |
SECRETION OF RECOMBINANT POLYPEPTIDES IN THE EXTRACELLULAR MEDIUM OF DIATOMS |
摘要 |
<p>The present invention concerns a transformed diatom comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide and (ii) a polypeptide, said heterologous signal peptide leading to the secretion of said polypeptide in the extracellular medium of said transformed diatom ; a method for producing a polypeptide which is secreted in the extracellular medium, said method comprising the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of said culture and (iii) purifying the secreted polypeptide in said extracellular medium; and use of said transformed diatom for the secretion of a polypeptide in the extracellular medium.</p> |
申请公布号 |
EP2630243(A1) |
申请公布日期 |
2013.08.28 |
申请号 |
EP20110787596 |
申请日期 |
2011.10.20 |
申请人 |
ALGENICS |
发明人 |
LEJEUNE, ALEXANDRE;MICHEL, REMY;CADORET, JEAN-PAUL;CARLIER, AUDE |
分类号 |
C12N15/82;C07K14/505;C07K14/55;C12P21/02 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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