发明名称 |
Composition for reprogramming somatic cells to generate induced pluripotent stem cells, comprising Bmi1 and low molecular weight substance, and method for generating induced pluripotent stem cells using the same |
摘要 |
Provided is a composition for reprogramming somatic cells to generate embryonic stem cell-like cells, comprising: a) a Bmi1 (B cell-specific Moloney murine leukemia virus integration site 1) protein or a nucleic acid molecule encoding the Bmi1 protein; and b) at least one low molecular weight substance selected from the group consisting of a set of a MEK/ERK (mitogen-activated protein kinase/extracellular regulated kinase) inhibitor and a GSK (glycogen synthase kinase) inhibitor, a set of a G9a HMTase (G9a histone methyltransferase) inhibitor and a DMNT (DNA methyltransferase) inhibitor, and a histone deacetylase inhibitor. Also, a method is provided for reprogramming somatic cells to generate embryonic stem cell-like cells using the composition. In addition to reducing the number of the reprogramming factors conventionally needed, the composition and method allow the generation of pluripotent embryonic stem cell-like cells which have high potential in the cell therapy of various diseases.
|
申请公布号 |
US8518700(B2) |
申请公布日期 |
2013.08.27 |
申请号 |
US201113103611 |
申请日期 |
2011.05.09 |
申请人 |
YOU SEUNGKWON;MOON JAI-HEE;KIM JUN SUNG;YOON BYUNG SUN;LEE JUNG HAN;JUN EUN KYOUNG;HEO JUNE SEOK;KIM JI HYUN;HWANG JI HYE;GWON SU HYUN;KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION |
发明人 |
YOU SEUNGKWON;MOON JAI-HEE;KIM JUN SUNG;YOON BYUNG SUN;LEE JUNG HAN;JUN EUN KYOUNG;HEO JUNE SEOK;KIM JI HYUN;HWANG JI HYE;GWON SU HYUN |
分类号 |
C12N15/85;C12N5/0735 |
主分类号 |
C12N15/85 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|