Method of providing portable biological testing capabilities

摘要

A method for providing portable biological testing capabilities free from biological contamination from an environment outside the device is provided. The method includes providing components configured to be assembled together to seal a volume against passage of biological materials between the volume and an environment outside the volume. The method further includes sterilizing the components and providing a sterilized culture medium. The method further includes assembling the components together with the sterilized culture medium within the volume. The method further includes sterilizing the assembled components by elevating the temperature. The method further includes flowing gas from within the volume to the environment while at an elevated temperature. The method further includes reducing the temperature to be less than the elevated temperature while preventing gas from flowing from the environment to the volume, thereby creating a pressure within the volume which is less than a pressure outside the volume.