| 摘要 |
PURPOSE: A method for preparing a foreign protein is provided to effectively remove a fusion tail from a fusion protein and to produce the foreign protein. CONSTITUTION: A method for preparing a foreign protein comprises: a step of preparing a first expression vector containing a gene encoding a soluble expression promoting protein and a gene encoding the foreign protein which are linked by a first linker; a step of preparing a second expression vector containing the gene encoding the soluble expression promoting gene and a gene encoding a proteolytic cleavage enzyme which are linked by a second linker; a step of transducing the first and second expression vectors into a host cell and preparing a transformant; and a step of culturing the transformant and cleaving the soluble expression promoting protein. The second linker is denoted by an amino acid sequence, DDDDK. The soluble expression promoting protein is LysRS or RBD. The foreign protein is BMP-2. The proteolytic cleavage enzyme is TEV. |
