| 摘要 |
FIELD: biotechnology.SUBSTANCE: synchronised daphnia is obtained from the most prolific clone of daphnia population, except for the laboratory population. The test samples are prepared. From the obtained daphnias the even-aged, parthenogenetic ones are selected, and concurrently the exposure is carried out to the test solution (test), in water (control), in the solution of the reference toxicant (K?) and in solution of mutagen (K?). The physiological condition in the original daphnia generation (F) is recorded. The toxicity factors are determined, namely, the death rate (L) and the fertility rate (P). The physiological condition of daphnias of the second generation (F) is recorded and the total mutagenic activity is determined according to the formula &=f/fwhere fis frequency of lethal, sublethal and morphological mutations in the experiment, and a fis frequency of lethal, sublethal and morphological mutations in the control with the subsequent comparing its value with isoeffective concentrations of reference mutagens (K?) and toxicants (K?) by release of limiting nuisance value and establishing the level of genotoxic activity on the basis of the results obtained.EFFECT: improving the accuracy of the estimate.2 dwg, 15 tbl, 4 ex |
