摘要 |
PURPOSE: A method for preparing yeast for producing 2,3-butanediol is provided to produce 50 to 100 times more 2,3-butanediol than a wild strain. CONSTITUTION: A method for preparing yeast for producing 2,3-butanediol comprises the step of inactivating one or more kinds of alcohol dehydrogenase selected from among alcohol dehydrogenase 1, alcohol dehydrogenase 3, and alcohol dehydrogenase 5. The step is conducted by mutating a nucleotide sequence for coding alcohol dehydrogenase through substitution, insertion, deletion, or a combination thereof. The efficiency of the yeast for producing 2,3-butanediol is 50 to 100 times greater than that of a wild strain of yeast. |