发明名称 |
DETECTION OF BIOLOGICAL MOLECULES BY DIFFERENTIAL PARTITIONING OF ENZYME SUBSTRATES AND PRODUCTS |
摘要 |
This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.
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申请公布号 |
US2013217041(A1) |
申请公布日期 |
2013.08.22 |
申请号 |
US201313738620 |
申请日期 |
2013.01.10 |
申请人 |
QUEEN'S UNIVERSITY AT KINGSTON;QUEEN'S UNIVERSITY AT KINGSTON |
发明人 |
BROWN R. STEPHEN;TABASH SAMIR P.;KOZIN IGOR S.;MARCOTTE ERIC J.P.;LEY ARTHUR N.;HALL KEVIN R.;HUSSAIN MOE;HODSON, SR. PETER V.;AKHTAR PARVEEN;BOWERS RAYMOND J.;WYNNE-EDWARDS ROBIN A.;ST. MARSEILLE JOHN G. |
分类号 |
G01N21/64;G01N33/569;C12M1/34;C12Q1/00;C12Q1/04;C12Q1/06;C12Q1/34;G01N21/77;G01N21/78;G01N33/573 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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