发明名称 |
ELECTROCHEMICAL ONE-STEP DETECTION METHOD OF DNA BASED ON THE TRANSPORT CONTROL OF SIGNALING MATERIALS |
摘要 |
<p>PURPOSE: An electrochemical one-step nucleic acid detection and quantitation method by controlling the transport of signaling materials is provided to simultaneously detect various kinds of target nucleic acids using an electrochemical one-step nucleic acid detection kit. CONSTITUTION: An electrochemical one-step nucleic acid detection and quantitation method by controlling the transport of signaling materials comprises the steps of: adding a target nucleic acid to a one-step nucleic acid detection kit and hybridizing the target nucleic, a capture probe, and a signaling probe; applying electric current to the kit and measuring a change of the electric current to detect and quantitate the target nucleic acid. The capture probe is fixed on the bottom surface of a reaction chamber and has a sequence which is complementary to a target nucleic acid sequence. The signaling probe is labeled with a signal substance which generates an electrochemical signal by reduction and oxidation and has a sequence which is complementary to another target nucleic acid sequence. The kit contains an analysis solution in which the signaling probe is dissolved and has a chamber with a working electrode, a reference electrode, and an auxiliary electrode. [Reference numerals] (AA) Metal nanoparticle-labeled signaling probe; (BB) Glass carbon electrode; (CC) Signal; (DD) Target nucleic acid; (EE) Gold matrix; (FF) Analysis chip</p> |
申请公布号 |
KR20130092164(A) |
申请公布日期 |
2013.08.20 |
申请号 |
KR20120013724 |
申请日期 |
2012.02.10 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
PARK, HYUN GYU;WON, BYOUNG YEON;BAEK, SONG YI |
分类号 |
C12Q1/68;C12Q1/48;G01N33/52 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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