发明名称 |
DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants |
摘要 |
Plant viral vectors have great potential in rapid production of proteins, but no simple. Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures (VLPs or full-size mAb) is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of this technology for producing multiple-subunit protein complexes.
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申请公布号 |
US8513397(B2) |
申请公布日期 |
2013.08.20 |
申请号 |
US200913060414 |
申请日期 |
2009.08.27 |
申请人 |
MASON HUGH S.;HUANG ZHONG;CHEN QIANG;ARNTZEN CHARLES J.;YUAN SHUO;HJELM BROOKE;THE ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OFARIZONA STATE UNIVERSITY |
发明人 |
MASON HUGH S.;HUANG ZHONG;CHEN QIANG;ARNTZEN CHARLES J.;YUAN SHUO;HJELM BROOKE |
分类号 |
C12N15/34;C12N5/10;C12N15/117;C12N15/13;C12N15/36;C12N15/82 |
主分类号 |
C12N15/34 |
代理机构 |
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