| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to microbiology. Disclosed is a method for quantitative determination of adhesion of Staphylococcus spp. to fibronectin and fibrinogen, during which the analysed staphylococcus culture is incubated in dimples of a polystyrene tray with separately protein-immobilised fibronectin, fibrinogen and in dimples in a liquid medium 199 for 60±15; further, bacteria that are not adhered are removed by washing three times with the medium 199 with tween 20 and the number of adhered staphylococci is determined, either by counting grown cells in the culture medium from optical density of the medium, or by counting cells grown on solid medium from a series of tenfold dilutions, or by determining activity of bacterial enzymes in adhered strains.EFFECT: owing to good reproducibility of results, high sensitivity, easier conduction of the reaction directly in dimples of the tray and the possibility of determining the factor of specific adhesion of strains to fibronectin and fibrinogen, the invention can be used in medical bacteriology for quantitative estimation of adhesive properties of staphylococci to human fibronectin (Fn) and fibrinogen (Fb) proteins, for characteristic of virulent properties in vitro based on the level of specific adhesion to said proteins.3 tbl, 3 ex |
