Process for the manufacture of oligonucleotides

摘要

A process for manufacturing an oligonucleotide which comprises removing beta-eliminating phosphorus-protecting groups, in particular beta-cyanoethyl protective groups from a protected oligonucleotide, wherein said removing comprises contacting the protected oligonucleotide with an amine solution in a solvent which preferably does not consist of pyridine, wherein the conjugate acid of the amine has preferably a pKa of greater than 11.5, and wherein the concentration of the amine in the solution is less than 0.5 mole/liters.