发明名称 |
Production of Metabolites |
摘要 |
A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
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申请公布号 |
US2013209613(A1) |
申请公布日期 |
2013.08.15 |
申请号 |
US201113700011 |
申请日期 |
2011.05.24 |
申请人 |
KATZ MICHAEL PATRIK;DURHUUS THOMAS;SMITS HANS PETER;FORSTER JOCHEN;FLUXOME SCIENCES A/S |
发明人 |
KATZ MICHAEL PATRIK;DURHUUS THOMAS;SMITS HANS PETER;FORSTER JOCHEN |
分类号 |
C12N15/81;A23K1/00 |
主分类号 |
C12N15/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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