摘要 |
<p>The purpose of the present invention is to provide a method and kit for detecting and quantifying a detection subject in a sample, capable of separating non-specific reactants from the sample in a short period of time. A method for detecting and quantifying a detection subject in a sample has steps for: mixing with the sample a conjugate combining a cohesive substance containing a stimuli-responsive substance and a particulate magnetic substance, and a substance with affinity for a substance other than the detection subject; separating the aggregated conjugate (A) by adding magnetic force to this mixture under conditions in which the stimuli-responsive substance aggregates; and detecting and quantifying the detection subject in the posterior mixture.</p> |