METHOD FOR QUANTIFYING TARGET SUBSTANCE

摘要

Provided is a method for quantifying a target substance typified by amino acid. This method includes: a step in which an enzyme capable of converting the target substance and also of generating a pyrophosphate using an adenosine tri-phosphate (ATP) as a base material therefor is caused to act on the target substance, and pyrophosphate is generated; a step in which a pyruvate phosphate dikinase (PPDK) is caused to act on the generated pyrophosphate, in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvic acid (PEP), and ATP, phosphoric acid, and pyruvic acid is generated; and a step in which the generated pyruvic acid is quantified. The amount of target substance is then determined on the basis of the obtained pyruvic acid amount. As a result of this invention, amino acid in a sample derived from an organism including a large quantity of a variety of impurities such as inorganic phosphoric acid and urea can be readily and quickly quantified without being affected by the impurities.