摘要 |
A protein tag is provided, which comprise a Proline-X-Proline (PXP) amino acid motif, wherein X is selected from any amino acid. Furthermore, fusion polypeptides and host cell comprising the proteintag is provided, as well as nucleic acids encoding these. Moreover, methods are provided of producing recombinant protein by culturing the host cell, expression a fusion polypeptide, and isolating the fusion polypeptide from the culture. |