发明名称 METHOD OF DETERMINING PRESENCE OF CELL SHOWING CHROMOSOME POLYPLOIDY AND HAVING CELL MASS CULTURING, USING IMAGE CYTOMETER
摘要 PROBLEM TO BE SOLVED: To provide a method of determining the presence of a cell related to high sensitivity, using an image cytometer, which can detect a cell showing chromosome polyploidy and having a cell mass culturing, in a cell group.SOLUTION: Using a method including a process (a) of bringing a fluorescent substance targeted at DNA and a cell sample originated in a cell group into contact with each other, a process (b) of measuring the DNA content of each cell in the cell sample, using an image cytometer, a process (c) of selecting a chromosome polyploidy cell, using an image cytometer, a process (d) of detecting the presence of cell growth of chromosome polyploidy cell, using an image cytometer, a process (e) of determining that the cell group contains a cell showing chromosome polyploidy and having cell mass culturing, in the case that the cell growth of the chromosome polyploidy cell is recognized, the presence of a cell showing chromosome polyploidy and having cell mass culturing, in the cell group, is determined by an image cytometer.
申请公布号 JP2013153704(A) 申请公布日期 2013.08.15
申请号 JP20120017988 申请日期 2012.01.31
申请人 YAMAGUCHI UNIV 发明人 KONDO TOMOKO;SASAKI ISANORI;ITO HIDEAKI
分类号 C12Q1/68;C12N15/09;C12Q1/02 主分类号 C12Q1/68
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