发明名称 Method of transfecting cells with nucleic acids using acidified polyethylenimine
摘要 An object of the present invention is to achieve a method capable of increasing transfection efficiency of polyethyleneimine (PEI) and reducing toxicity thereof, to provide a PEI with high transfection efficiency, and to provide a method of introducing nucleic acids into cells comprising using the PEI. There are provided a method of introducing nucleic acids into cells comprising mixing acidified PEI with nucleic acids under acidic conditions and applying the resulting mixture to cells, a reagent for introducing nucleic acids into cells comprising an acidified PEI solution, and a reagent kit including the solution.
申请公布号 US8507270(B2) 申请公布日期 2013.08.13
申请号 US20100752188 申请日期 2010.04.01
申请人 YAMAGUCHI NAOTO;FUKUMOTO YASUNORI;NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY 发明人 YAMAGUCHI NAOTO;FUKUMOTO YASUNORI
分类号 C12N15/00 主分类号 C12N15/00
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