发明名称 |
Methods and kits for reducing non-specific nucleic acid amplification |
摘要 |
Methods and kits for efficient amplification of nucleic acids are provided. The methods comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having terminal mismatch primer-dimer structure. The methods also comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having nucleotide analogues. The methods enhance efficiency of nucleic acid amplification reaction by reducing non-specific amplification reactions.
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申请公布号 |
US8507662(B2) |
申请公布日期 |
2013.08.13 |
申请号 |
US201113183480 |
申请日期 |
2011.07.15 |
申请人 |
NELSON JOHN RICHARD;DUTHIE ROBERT SCOTT;SHAH SONALI JAGDISH;SMITH CLIFFORD LESLIE;GENERAL ELECTRIC COMPANY |
发明人 |
NELSON JOHN RICHARD;DUTHIE ROBERT SCOTT;SHAH SONALI JAGDISH;SMITH CLIFFORD LESLIE |
分类号 |
C07H21/04;C12N9/00 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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