The primers for differentiation of O, A, and Asia 1 serotypes of foot and mouth disease virus and multiplex RT-PCR method

摘要

PURPOSE: A primer for diagnosing foot-and-mouth disease type O, type A, and type Asia 1 viruses and a multiplex RT-PCR method using the same are provided to simultaneously diagnose three kinds of serotypes through one-step RT-PCR. CONSTITUTION: A primer set for diagnosing foot-and-mouth type O virus has VN-VP1R forward primer of sequence number 1 and VN-OF reverse primer of sequence number 2. A primer set for diagnosing foot-and-mouth disease type Asia 1 virus contains VN-VP1R forward primer of sequence number 1 and VN-As1F reverse primer of sequence number 3. A primer set for diagnosing foot-and-mouth type A virus contains VN-VP1R forward primer of sequence number 1 and AN-AF reverse primer of sequence number 4.