| 摘要 |
The present invention relates to amplification oligonucleotides (primers) and detection probes for the detection of human immunodeficiency virus type-1 (HIV-1) in a test sample. The primers and probes may be used in an assay for the detection and/or quantitation of HIV-1 nucleic acid. In various embodiments, the primers and detection probes may be used for real-time reverse transcription polymerase chain reaction (RT-PCR) assays for detection of HIV-1 nucleic acid. The detection probes may, for example, be fluorescently-labeled TaqMan® probes. In one embodiment, amplification primers and probes may be specific for the pol region of the HIV-1 genome. In another embodiment, the amplification primers and probes may be specific for the env region of the HIV-1 genome. "Cocktails" of multiple amplification primers and/or probes may be used to achieve maximal coverage of different HIV-1 subtypes. |
