发明名称 |
METHOD FOR DETECTING NON-TRANSFERRIN BOUND IRON |
摘要 |
A method of quantifying non-transferrin bound iron (NTBI) in a biological fluid is provided, the method comprising: (a) contacting a sample of the biological fluid with a reducing agent to obtain redox-active iron; (b) contacting the sample of the biological fluid with a nitrilotriacetate (NTA) at a concentration of 0.4-2 mM to mobilize non-transferrin bound iron including redox inactive and the redox active iron in the sample; (c) contacting the sample of the biological fluid of step (b) with an indicator including an iron binding moiety and fluorophore; and (d) detecting and quantifying a fluorescent signal of the fluorophore thereby quantifying the NTBI in the biological sample.
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申请公布号 |
US2013203800(A1) |
申请公布日期 |
2013.08.08 |
申请号 |
US201213644565 |
申请日期 |
2012.10.04 |
申请人 |
CABANTCHIK IOAV ZVI;BREUER WILLIAM;YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREWUNIVERSITY OF JERUSALEM LTD. |
发明人 |
CABANTCHIK IOAV ZVI;BREUER WILLIAM |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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