摘要 |
PURPOSE: A method for manufacturing antibiotic produced from Bacillus amyloliquefaciens GYL4 is provided to obtain eco-friendly germicide composition with excellent biodegradation property and to prevent cucumber anthracnose. CONSTITUTION: A Bacillus amyloliquefaciens GYL4(deposit number: KACC91655P) has an antifungal activity against plant pathogenic fungi such as Fusarium oxysporum f.sp. lycopersici, Fusarium oxysporum f.sp. lycopersici JCM12575, Colletotrichum cocodes, Colletotrichum gloeosporioides, Colletotrichum orbiculare, or Cylindrocarpon destructans. A method for manufacturing antibiotics with the antifungal activity comprises: a step of extracting a Bacillus amyloliquefaciens GYL4 fungus body, a spore, a mixture thereof, or a culture medium with butanol; and a step of isolating and purifying bacillomycin D1 from the butanol extract. |