| 摘要 |
The invention relates to the field of biotechnologies, in particular to the method of extraction and purification of nucleic acids. The technical problem of the claimed solution is to increase the output and processability of the process of extraction and purification of nucleic acids, increase the process rate, decrease the number of performed operations, increase the purity of obtained nucleic acids, increase the sorption properties of a vessel in which the process being performed, providing uniform coating throughout the whole vessel surface both on large and limited areas, increase the optical transparency of the vessel. The problem is solved by providing a vessel of plastic for nucleic acids sorption from liquid medium comprising silicon oxide coating on inner surface of the vessel, representing a nano-coating (defined as a coating with the thickness of from 1 to 100 nm), formed by a thin- film synthesis. The method of extraction and purification of nucleic acids from liquid medium comprises the sorption thereof on inner walls of a vessel, rinsing out of admixtures and elution in a saline solution, heating the vessel, wherein as a sorbent there is used silicon oxide SiO2 nano-coating formed by a thin-film synthesis in a vessel, wherein the vessel is further added with chaotropic agent, pH-buffer, the vessel after adding the elution solution is heated up to 95°C to extract DNA or up to 65°C to extract RNA, thoroughly agitated and the nucleic acids solution is taken out into another vessel. Besides, there is used silicon oxide nano-coating with the size of from 1 to 25 nm. The embodiments are given. |
