Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
申请公布号
US2013196436(A1)
申请公布日期
2013.08.01
申请号
US201313794654
申请日期
2013.03.11
申请人
CELLULAR DYNAMICS INTERNATIONAL, INC.;CELLULAR DYNAMICS INTERNATIONAL, INC.
发明人
STANKEWICZ CASEY;RILEY MATT;BEARDSLEY NATHANIEL;WANG WEN BO;FUHRKEN PETER;KATTMAN STEVEN