发明名称 Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton
摘要 In one aspect, the present application discloses a chimeric gene comprising (a) a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80% sequence identity thereto any of which confers stress inducibility on said chimeric gene; (b) a second nucleic acid sequence encoding an expression product of interest, which is involved in the response of a cotton plant to stress; and optionally (c) a transcription termination and polyadenylation sequence. In another aspect, the application discloses a cotton plant cell comprising (a) a chimeric gene comprising a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80 sequence identity thereto any of which confers stress inducibility on said chimeric gene; (b) a second nucleic acid sequence encoding an expression product of interest; and optionally (c) a transcription termination and polyadenylation sequence. In addition, the present application discloses a cotton plant, a method of expressing a transgene in cotton under stress conditions, a method of producing a cotton plant, a method of detecting the expression of a transgene under stress conditions and a method for modulating the resistance of a cotton plant to stress as characterized in the claims.
申请公布号 AU2012210618(A1) 申请公布日期 2013.08.01
申请号 AU20120210618 申请日期 2012.01.24
申请人 BAYER CROPSCIENCE N.V. 发明人 PIEN, STEPHANE;DEN BOER, BART
分类号 C12N15/82;A01H5/00 主分类号 C12N15/82
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