摘要 |
PROBLEM TO BE SOLVED: To provide a manufacturing method of a biochip used for detection and analysis of a number of protein in a biological sample, and capable of performing highly sensitive, high-throughput detection of a physiologically active substance in which protein is immobilized at an arbitrary position on the surface of a substrate, and unnecessary adsorption and connection of a physiologically active substance is suppressed without coating an adsorption preventing agent.SOLUTION: Provided is a manufacturing method of a biochip in which protein is immobilized on the surface of a substrate, the method comprising the steps of: (1) applying a polymer having a polyethylene glycol group on the surface of a substrate; (2) performing spot application of or applying a liquid in which protein is dissolved or dispersed in a solvent on the surface of the substrate; (3) and immobilizing the protein. Also provided is a biochip manufactured by the manufacturing method. |