发明名称 |
DIRECT CAPTURE, AMPLIFICATION AND SEQUENCING OF TARGET DNA USING IMMOBILIZED PRIMERS |
摘要 |
Certain embodiments provide a method for capturing a genomic fragment. The method may comprise: obtaining a substrate comprising a first population of surface-bound oligonucleotides and a second population of surface-bound oligonucleotides; hybridizing a first member of the first population of surface-bound oligonucleotides to a selection oligonucleotide comprising a region that hybridizes with the first member and a region that contains a genomic sequence; extending the first member of the first population of surface-bound oligonucleotides to produce a support-bound selection primer that comprises a sequence that is complementary to the genomic sequence; hybridizing the support-bound selection primer to a nucleic acid fragment comprising the genomic sequence; extending the support-bound selection primer to produce an extension product that contains a sequence that flanks the genomic sequence, e.g., in a genome; and amplifying the extension product on the substrate. |
申请公布号 |
EP2619329(A1) |
申请公布日期 |
2013.07.31 |
申请号 |
EP20110827484 |
申请日期 |
2011.09.21 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
MYLLYKANGAS, SAMUEL;BUENROSTRO, JASON;JI, HANLEE P. |
分类号 |
C12Q1/68;C40B40/06 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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