| 摘要 |
<p>A region in which many fluorescent substances are present is identified without carrying out a complicated calculation even if a fluorescence intensity has changed due to a distance and an angle. Provided is a fluorescence observation device (1) comprising an illuminating section (3) that emits excitation light and illumination light onto a subject (A); a fluorescence-image capturing section (18) that acquires a fluorescence image (S 2 ) by image-capturing fluorescence generated in the subject (A) by irradiation with the excitation light from the illuminating section (3); a return-light-image capturing section (17) that acquires a return-light image (S 1 ) by image-capturing return light that returns from the subject (A) by irradiation with the illumination light from the illuminating section (3); a region extracting section (24) that extracts a high-luminance region in which the fluorescence image (S 2 ) acquired by the fluorescence-image capturing section (18) has a gradation level higher than or equal to a set threshold value (S 4 ); and a threshold setting section (22) that sets the threshold value (S 4 ) higher as the gradation level of the return-light image (S 1 ) acquired by the return-light-image capturing section (17) increases.</p> |
