| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to the technology of producing biological preparations and can be used in the pharmaceutical industry. The method of preparing a pegylated human growth hormone involves pegylation of said protein with double-stranded polyethylene glycol in one site at pH not lower than 6.0. The obtained pegylated growth hormone is subjected to electrophoresis in polyacrylamide gel in non-reducing conditions, and separating the end product from a band which corresponds to a lower apparent molecular weight. The method, which involves pegylation of the human growth hormone at pH not lower than 8.0 and separating the obtained product, enables to obtain a preparation mainly containing pegylated human growth hormone with a lower apparent molecular weight. The pegylated growth hormone with a lower apparent molecular weight and a preparation rich in said hormone are used to prepare a medicament for treating diseases that require treatment with a growth hormone.EFFECT: use of the invention increases biological activity of the growth hormone and prolongs its metabolic half-life.19 cl, 24 dwg, 3 tbl, 6 ex |
