发明名称 |
NON-INVASIVE DETECTION OF FETAL GENETIC TRAITS |
摘要 |
Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains @500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising @500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.
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申请公布号 |
US2013190483(A1) |
申请公布日期 |
2013.07.25 |
申请号 |
US201313779300 |
申请日期 |
2013.02.27 |
申请人 |
SEQUENOM, INC.;SEQUENOM, INC. |
发明人 |
HAHN SINUHE;HOLZGREVE WOLFGANG;ZIMMERMANN BERNARD;LI YING |
分类号 |
C12Q1/68;G01N33/53;B01J20/281;C12N15/09;G01N30/88;G01N37/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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