发明名称 |
METHODS TO MUTATE CIRCULAR DEOXYRIBONUCLEOTIDES |
摘要 |
The invention provides improved mutagenic plasmids and a method for making and using them. In one embodiment a cyclic mutagenic nucleic acid is replicated to form interlocking, digestibly separable duplex or simplex rings; and then one or more of the interlocked rings are cleaved selectively by means of a restriction enzyme to liberate an intact duplex or simplex ring. The mutagenic plasmids show substantially improved efficiencies in transforming cellular microorganism that are exposed to them. |
申请公布号 |
WO2013109950(A1) |
申请公布日期 |
2013.07.25 |
申请号 |
WO2013US22237 |
申请日期 |
2013.01.18 |
申请人 |
KENNESAW STATE UNIVERSITY RESEARCH AND SERVICES FOUNDATION;SALERNO, JOHN, C. |
发明人 |
SALERNO, JOHN, C. |
分类号 |
C07H21/04;C12N15/11;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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