IMMUNOASSAY OF MYOGLOBIN USING SPFS (SURFACE PLASMON FIELD-ENHANCED FLUORESCENCE SPECTROSCOPY)

摘要

PROBLEM TO BE SOLVED: To provide means capable of highly sensitively quantitatively determining or detecting myoglobin in a sample.SOLUTION: An immunoassay of myoglobin in a sample includes the steps of: forming a sandwich type immune complex including solid-phased anti-myoglobin antibody, myoglobin, and fluorescence-labeled anti-myoglobin antibody; and measuring fluorescence intensity emitted from a fluorescent material included in the formed sandwich type immune complex by an SPFS (Surface Plasmon field-enhanced Fluorescence Spectroscopy).