THE ALTERATION OF SIGNAL TRANSDUCTION BY APPLYING ENDORPHIN NEURONAL CELL LINE INCLUDING SHSY5Y CELL LINE WITH INCREASED HSA-MIR-708 MIRNA EXPRESSION

摘要

PURPOSE: A method for increasing the expression of hsa-miR-708 miRNA and altering a signal transduction system by treating neuronal cells with endorphin is provided to show the same effect as endorphin by adding an antagomer or miRNA using hsa-miR-708 miRNA. CONSTITUTION: A method for mimicking the effect of endorphin and a method for supplementing a specific signal comprise the step of directly treating a neuronal cell line including in vitro cultured SHSy5y cells with hsa-miR-708 miRNA or treating the cell line with an antagomer. A method for promoting the expression of proteins comprises the steps of treating the cell line with endorphin and reducing the expression level of hsa-miR-708. The proteins are gon-4-like (C. elegans), KIAA0355, forkhead box J3, nuclear factor (erythroid-derived 2)-like 2, cyclin-dependent kinase 2B-inhibitor-related protein, arsenic (+3 oxidation state) methyltransferase, jumonji domain containing 6, engrailed homeobox 2, glycoprotein M6A, and chromosome 14 open reading frame 101. When endorphin is added, hsa-miR-708 miRNA is reduced and the expression level of the proteins is increased by suppressing the targeting of mRNA of a specific molecule.