METHOD FOR THE PRODUCTION OF EVOLVED MICROORGANISMS WHICH PERMIT THE GENERATION OR MODIFICATION OF METABOLIC PATHWAYS

摘要

Strain (A) of a microorganism which produces a 2-amino-4-alkylthio-butyric acid (I) by metabolizing a simple sugar and a thiol (II), or its salt, and has at least one gene encoding an enzyme with modified methionine synthase (MS) activity. Strain (A) of a microorganism which produces a 2-amino-4-alkylthio-butyric acid of formula (I) RS-(CH 2) 2-CHNH 2-COOH (I) by metabolizing a simple sugar and a thiol (II), R'-SH, or its salt, and has at least one gene encoding an enzyme with modified methionine synthase (MS) activity. R : 1-8C linear or branched alkyl, optionally substituted by one or more hydroxy or by (hetero)aryl with one or more nitrogen or sulfur atoms in the ring, i.e. phenyl, pyridyl, pyrroly, pyrazolyl, triazolyl, tetrazolyl, thiazolyl or thienyl; R' : hydrogen or as R. Independent claims are also included for the following: (1) modified cystathioninegamma -synthase (X) having MS activity; (2) nucleic acid (NA) that encodes (X); (3) cloning and/or expression vector containing (NA) and elements for regulating expression and transcription of (X); (4) method of screening for a bacterial strain that has a gene encoding (X) or an acylhomoserine sulfhydrylase (Y), for preparation of (A); and (5) method for producing (I) by culturing (A) or by using MS activity present in activated bacteria or their extracts.