发明名称 |
ASSAY FOR DETECTING CLOSELY- RELATED SEROTYPES OF HUMAN PAPILLOMAVIRUS (HPV) |
摘要 |
A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected. |
申请公布号 |
EP2536853(A4) |
申请公布日期 |
2013.07.24 |
申请号 |
EP20110745156 |
申请日期 |
2011.02.16 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
CHEN, CHI;PECK, HUGH J.;PORTER, MICHAEL;RICHART, GREGORY A.;MCMILLIAN, RAY A. |
分类号 |
C12Q1/68;C12N15/11;C12N15/37;C12Q1/70 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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