| 摘要 |
A chemiluminescent enzyme immunoassay method for quantifying antigen or antibody using 1,1'-oxalyldiimidazole (ODI) derivative or 1,1'-oxalyldisodium benzoate (ODB) derivative chemiluminescence (CL) detection was developed. Also, various enzymes were quantified using ODI derivative or DOB derivative CL detection. Fluorescent compound formed from a substrate (non-fluorescent compound) through the enzyme assay methods emitted CL when the fluorescent compound received energy from high-energy intermediate formed in ODI derivative or ODB derivative CL reaction.
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