METHOD FOR PREPARING SETARIA ANTIGEN FOR DIAGNOSIS OF DOG'S DIROFILARIASIS

摘要

FIELD: medicine.SUBSTANCE: invention refers to veterinary science and concerns a method for preparing a Setaria antigen. The method comprises homogenising the mature helminthes Setaria labiato-papillosa manually in the cold, extracting homogenated proteins in phosphate-buffered saline pH 7.2-7.4 in ratio 1:10 for 48 hours at +4°C in a magnetic mixer, centrifuging the homogenate and collecting a supernatant at 15 thousand rpm and +4°C for 20 minutes; the extracted supernatant is additionally purified by gel chromatography in a Superose 12 Prep Grade column at flow rate of sodium phosphate buffer (pH 6.0) - 30 ml/h; the antigen-active fractions are collected by ELISA assay with using dog's dirofilariasis positive and negative reference serums.EFFECT: fractions containing the antigen-active protein components and having shown the best results with reference serums are combined, measured for the protein content and used as an antigen for ELISA assay for diagnosis of dog's dirofilariasis.