Real-time measure of a population of nucleic acids, in particular by PCR

摘要

The invention relates to a method for measuring the ratio of nucleic acids of a target sequence in a sample of interest. The method comprises a plurality of steps, in particular: submitting the sample of interest to an amplification processing in the presence of at least one nucleic-acid marker specific to said target sequence; measuring a physical value representative of the variation in the marker; calculating a parameter F