| 摘要 |
The present invention provides methods, devices and test kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing solid nutrient media. The invention includes mixing the one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on the one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on the one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane, and detection and identification of the one or more target microorganisms is achieved in less than one hour.
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