发明名称 |
NUCLEOTIDES AND APTAMERS CONTAINING BORONIC ACID GROUPS HAVING BIASED BINDING TO GLYCOSYLATED PROTEINS, AND USES THEREOF |
摘要 |
The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein.
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申请公布号 |
US2013184160(A1) |
申请公布日期 |
2013.07.18 |
申请号 |
US20080669593 |
申请日期 |
2008.07.17 |
申请人 |
WANG BINGHE;LI MINYONG;HUANG ZHEN;LIN NA |
发明人 |
WANG BINGHE;LI MINYONG;HUANG ZHEN;LIN NA |
分类号 |
C12N15/115;C07H23/00;C12N15/10;G01N33/68;G01N33/86 |
主分类号 |
C12N15/115 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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