发明名称 |
NUCLEOTIDES AND APTAMERS CONTAINING BORONIC ACID GROUPS HAVING BIASED BINDING TO GLYCOSYLATED PROTEINS, AND USES THEREOF |
摘要 |
<p>The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein.</p> |
申请公布号 |
EP2176277(A4) |
申请公布日期 |
2013.07.17 |
申请号 |
EP20080781954 |
申请日期 |
2008.07.17 |
申请人 |
GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC. |
发明人 |
WANG, BINGHE;LI, MINYONG;HUANG, ZHEN;LIN, NA |
分类号 |
C07H19/00;A61K48/00;C07H19/04;C07H21/00;C12Q1/68 |
主分类号 |
C07H19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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